1 - Nutrition & Metabolism Department, NOVA Medical School | Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, Lisboa, Portugal.
2 - CHRC - Comprehensive Health Research Centre, NOVA Medical School | Faculdade de Ciências Médicas (NMS|FCM), Universidade Nova de Lisboa, Lisbon, Portugal.
3 - Nutrition & Metabolism Department, CINTESIS@RISE, NOVA Medical School | Faculdade de Ciências Médicas (NMS|FCM), Universidade Nova de Lisboa, Lisboa, Portugal.
4 - Neonatal Intensive Care Unit, Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central, Lisboa, Portugal.
5 - Medicine of Woman, Childhood and Adolescence academic area, NOVA Medical School | Faculdade de Ciências Médicas (NMS|FCM), Lisbon, Portugal
- Microorganisms 2023;11(10):2482. doi: 10.3390/microorganisms11102482
Early-life gut dysbiosis has been associated with increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who undergone intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. First effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria amount (+21%, p=0.026), a decrease in Staphylococcus (-21%, p=0.002) and Candida spp (-16%, p=0.045), and an increase in Lactobacillus (+3%, p=0.045) and in less abundant genera belonging to the Enterobacteriales family were found. An increase in alpha-diversity (Shannon’s and Simpson’s indexes) and significant al-terations in beta-diversity were observed. A correlation of necrotizing enterocolitis with higher Staphylococcus abundance and higher alpha-diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria amount. In conclu-sion, these results suggest that positive changes occur in gut microbiota profile of infants three weeks after intestinal surgery.
Palavras-Chave: enterostomy; infants; microbiota; necrotizing enterocolitis; prematurity