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2020

ANUÁRIO DO HOSPITAL
DONA ESTEFÂNIA

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COLONISATION OF THE PROXIMAL INTESTINAL REMNANT IN NEWBORN INFANTS WITH ENTEROSTOMY: A LONGITUDINAL STUDY PROTOCOL

Inês Barreiros Mota1,2, Cláudia Marques1,2, Ana Faria1-3, Maria Teresa Neto4,5, Gonçalo Cordeiro-Ferreira4, Daniel Virella4,6, Ana Pita4, Luís Pereira-da-Silva4-6, Conceição Calhau1,2,7

1- Nutrition and Metabolism, NOVA Medical School | Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisbon, Portugal
2- CINTESIS - Center for Health Technology and Services Research, Porto, Portugal
3- Comprehensive Health Research Centre, Universidade NOVA de Lisboa, Lisbon, Portugal
4- Neonatal Intensive Care Unit, Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central, Lisbon, Portugal
5- Medicine of Woman, Childhood and Adolescence, NOVA Medical School | Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Lisbon, Portugal
6- Research Unit, Centro Hospitalar Universitário de Lisboa Central, Lisbon, Portugal
7- Unidade Universitária Lifestyle Medicine, José de Mello Saúde by NOVA Medical School, Lisbon, Portugal

- BMJ Open 2019;9:e028916

Introduction: The gut microbiota plays a main role in the maintenance of host’s health. Exposure to different conditions in early life contributes to distinct ‘pioneer’ bacterial communities in the intestine, which shape the newborn infant development. Newborn infants with congenital malformations of the gastrointestinal tract (CMGIT), necrotising enterocolitis (NEC) and spontaneous intestinal perforation (SIP) commonly require abdominal surgery and enterostomy. The knowledge about the colonisation of these newborns’ intestine by microorganisms is scarce. This protocol is designed to explore the microbial colonisation over time of the proximal intestinal remnant in newborn infants who underwent surgery for CMGIT, NEC or SIP and require enterostomy.
Methods and analysis: The literature about microbiota colonisation in newborn infants with enterostomy was reviewed and an observational, longitudinal, prospective study was designed. The infants will be recruited at the Neonatal Intensive Care Unit of the Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central. Samples of the enterostomy effluent will be collected every 3 days, through 21 days after the first collection. The microorganisms colonising the proximal intestinal remnant will be identified using the 16S rRNA sequence analysis and a subset of microorganisms will be quantified using real-time PCR. This protocol may serve as basis for future observational and interventional studies on the modulation of the intestinal microbiota (eg, probiotics) on short and long-term outcomes in this population.
Ethics and dissemination: This study protocol was approved by the Ethics Committee of Centro Hospitalar Universitário de Lisboa Central (441/2017) and by the Ethics Committee of NOVA Medical School, Universidade Nova de Lisboa (n°50/2018/CEFCM). The results will be spread through peer-reviewed publications and presentations at international scientific meetings.

Trial registration number NCT03340259.

Palavras Chave: congenital malformations of the gastrointestinal tract, enterostomy, gut colonization, microbiota, necrotising enterocolitis, newborn infant